effect of zinc nano-complex on bull semen quality after freeze-thawing process
نویسندگان
چکیده
the effect of zinc nano-particles (zn- nano- complex) on bull sperm quality after freeze-thawing process studied. ejaculates collected from four holstein bulls twice a week. on the day of semen collection, four ejaculates were pooled and diluted with bioxcell extender containing 0, 10-6, 10-5, 10-4, 10-3, 10-2 molar of zinc nano-complex and frozen. after thawing, sperm motility and motion parameters, plasma membrane integrity, abnormal morphology, plasma membrane functionality and mitochondrial activity were determined. the proportion of the total and progressive motile sperm, the plasma membrane integrity and proportion of the spermatozoa with abnormal morphology was not different among groups. zinc nano-complex groups represented a higher plasma membrane functionality than that of control group. moreover, our flowcytometric data suggested that spermatozoa in the groups of zinc nano-complex possessed higher mitochondrial activity as compared with the control group. mitochondrial activity in 10-2 was higher than the 10-6 group. in conclusion, supplementation of zn nano-complex, can improve the plasma membrane functionality and mitochondrial activity of bull spermatozoa in a dose dependent manner without any deleterious effect on motility parameters.
منابع مشابه
Effect of various levels of catalase antioxidant in semen extenders on lipid peroxidation and semen quality after the freeze-thawing bull semen
The objective of this study was to evaluate effect of different concentrations of catalase in two extenders on motility, viability and lipid peroxidation bull spermatozoa during semen freezing process. Thirty ejaculates collected from ten Holstein bulls were pooled and evaluated at 37 °C. Pool ejaculated was split into two main experimental groups, 1 and 2. In experiment 1, specimen was dilute...
متن کاملeffect of zinc nano-complex on bull semen quality and pregnancy outcome
this study aimed to investigate the effect of different concentration of zinc nano-complex on bull spermatozoa quality and pregnancy outcome after freeze-thawing process. ejaculates were collected from four holstein bulls, twice a week during four weeks. semen samples diluted with bioxcell extender containing zero (control), 10-6, 10-5, 10-4, 10-3 and 10-2 m of zinc nano-complex. the samples we...
متن کاملEffect of Antioxidant D-Aspartic Acid and Thawing Rate on the Freeze-Thawing Process of Ram Semen
The study was conducted to determine the influence of D-aspartic acid (D-Asp) as antioxidant supplement and thawing rates on ram sperm motility, membrane integrity, abnormality, viability, mitochondria activity, malondialdehyde and antioxidant activities, and total antioxidant capacity after freezing-thawing process. Semen samples from five mature rams (3-4 years old) were diluted with extender...
متن کاملEffect of various levels of catalase antioxidant in semen extenders on lipid peroxidation and semen quality after the freeze-thawing bull semen
The objective of this study was to evaluate effect of different concentrations of catalase in two extenders on motility, viability and lipid peroxidation bull spermatozoa during semen freezing process. Thirty ejaculates collected from ten Holstein bulls were pooled and evaluated at 37 °C. Pool ejaculated was split into two main experimental groups, 1 and 2. In experiment 1, specimen was diluted...
متن کاملEffect of curcumin on rat sperm morphology after the freeze-thawing process
Reactive oxygen species (ROS) generation, induced by the cryopreservation process, can be responsible for mammalian sperm damage. Curcumin is known as an effective antioxidant against oxidative stress. The aim of this study was to evaluate the effects of curcumin on sperm count, motility and viability, semen total antioxidant capacity and DNA integrity of rat spermatozoa during semen freeze-tha...
متن کاملeffect of various levels of catalase antioxidant in semen extenders on lipid peroxidation and semen quality after the freeze-thawing bull semen
the objective of this study was to evaluate effect of different concentrations of catalase in two extenders on motility, viability and lipid peroxidation bull spermatozoa during semen freezing process. thirty ejaculates collected from ten holstein bulls were pooled and evaluated at 37 °c. pool ejaculated was split into two main experimental groups, 1 and 2. in experiment 1, specimen was diluted...
متن کاملمنابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
تولیدات دامیجلد ۱۷، شماره ۲، صفحات ۳۷۱-۳۸۰
کلمات کلیدی
میزبانی شده توسط پلتفرم ابری doprax.com
copyright © 2015-2023